C-terminal half of tetanus toxin fragment C is sufficient for neuronal binding and interaction with a putative protein receptor
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منابع مشابه
C-terminal half of tetanus toxin fragment C is sufficient for neuronal binding and interaction with a putative protein receptor.
Tetanus neurotoxin (TeNT) is a powerful bacterial protein toxin that cleaves VAMP/synaptobrevin, an essential protein of the synaptic vesicle fusion machinery, and consequently blocks neurotransmission. The extreme neurospecificity of TeNT is determined by the binding of its C-terminal domain (fragment C or H(C)) to neuronal receptors. Whereas polysialogangliosides are known acceptors for the t...
متن کاملCloning and expression of tetanus toxin C fragment (Fc) in prokaryotic vector for constructing recombinant protein based vaccine for tetanus
Tetanus is a disease caused by tetanus toxin, a potent inhibitor for the release of inhibitory neurotransmitter in the central nervous system that causes spastic paralysis. Fragment C (52 kD) of this toxin is responsible for binding to the neuronal membrane. For this reason, and also its non toxigenic and immunogenic nature, this fragment might be ideal for new vaccine development. Presently, w...
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The non-toxic C fragment of tetanus toxin fused to the beta-galactosidase enzyme was analysed as a neuroanatomical tracer. After intramuscular injection in rat tongue, its location in the hypoglossal network was compared with other classic tracers such as neurotropic viruses. The hybrid protein reached second and higher-order neurons after crossing several synapses. It appears to be a powerful ...
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Clostridium Botulinum Type E neurotoxin heavy chain consists of two domains: the translocation domain asthe N-terminal half and the binding domain as the Cterminal half (Hc). One effective way to neutralize botulinum neurotoxin is to inhibit binding of this toxin to neuromuscular synapses with antibodies against binding domain. Two synthetic genes, coding for Hc (the full length binding d...
متن کاملcloning and expression of tetanus toxin c fragment (fc) in prokaryotic vector for constructing recombinant protein based vaccine for tetanus
tetanus is a disease caused by tetanus toxin, a potent inhibitor for the release of inhibitory neurotransmitter in the central nervous system that causes spastic paralysis. fragment c (52 kd) of this toxin is responsible for binding to the neuronal membrane. for this reason, and also its non toxigenic and immunogenic nature, this fragment might be ideal for new vaccine development. presently, w...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 2000
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3470199